BIOTECHNOLOGICAL STRATEGIES APPLIED TO ANIMAL PRODUCTION AND HEALTH

CECILIA LIAUDAT, A.; CAPELLA, VIRGINIA; BONINO, R.; SOSA, E.; SOMMARO, ANDRÉS; GONZÁLEZ, M. A.; OPIZZO, BIANCA ANA; BLOIS, DAMIAN; MORILLA, GRICELDA; FILI, ALEJANDRO; BARBERO, CÉSAR A.; RIVAROLA, CLAUDIA R.; BOSCH, PABLO; RODRIGUEZ, NANCY

Congreso; XL Annual Meeting, Sociedad de Biología de Cuyo; 2022

Tissue engineering is an emerging interdisciplinary field whose purpose is to replace or regenerate a damaged organ through the use of biomaterials. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are synthetic polymeric materials with innate chemical and mechanical similarity to the extracellular matrix. Due to this, these scaffolds are being studied to be applied in tissue regeneration to support the growth of different cell types. Studies carried out in our laboratory evaluated the effect that PNIPAM and PNIPAM copolymers produce on of immunological, fibroblastic, renal and pulmonary cells biocompatibility. The results showed that hydrogels are biocompatible with all cell lines analyzed without activating an inflammatory immune response. In addition, PNIPAM hydrogels were used as sperm selection surfaces for livestock species, where it was possible to obtain a bovine and swine sperm selection system with high percentages of viability and motility. On the other hand, preliminary results showed that the addition of calcitriol to sperm medium acts as a natural capacitation agent. Also, the analysis of biological characterization of bovine oocytes from slaughterhouse is an important step to define its use in assisted reproduction techniques.