Adenoviral transduction of mesenchymal stem cells

BOSCH P; STICE SL

Stem Cells Assays. Methods in Molecular Biology Series Vol. 407

The Human Press Inc.

Lugar: Totowa, NJ; Año: 2007; p. 265 - 274

Intact or genetically manipulated mesnechymal stem cells (MSCs) are being considered an important cell source for developing human cell-based therapeutic approaches. For applications in which transient, high level expression of the transgene is necessary, adenovirus vectors have become increasingly popular gene transfer vehicles. However, host range and cell-type tropism restrict the use of specific adenovectors, sometimes necessitating the lengthy development of vectors with appropriate cell specificity. Here we present a versatile and inexpensive porcine MSC transduction procedure that can also be used on other cell types from various species, including human that are otherwise refractory to adenovirus infection.