INVESTIGADORES
PIGOZZI Maria Ines
congresos y reuniones científicas
Título:
Basic and practical aspects of immunofluorescent labeling in meiosis
Autor/es:
PIGOZZI MI
Lugar:
Atibaia
Reunión:
Taller; 4ta Reunión Brasileña de Citogenética; 2015
Resumen:
Among mammals, the meiotic prophase I
was initially studied using thin sections and microspread meiotic nuclei at the
electron microscope. These methods relied on the visualization of the
synaptonemal complexes which reproduce the chromosome behavior during early
meiotic stages. For many years, the technically challenging procedures and the requirement
of expensive equipment kept synapsis and recombination studies limited to
relatively few laboratories worldwide. The knowledge on the molecular basis of
chromosome synapsis and recombination in yeast was rapidly followed by a
renewed interest in mutant mice causing infertility and the generation of
meiotic knock-out organisms. This interest boosted the production of antibodies
against meiotic axes from mammals and the use of these antibodies in immunofluorescence
paved the way to study the behavior of chromosomes during prophase and the role
of their associated proteins in higher eukaryotes. The goal of the course is to
provide an introduction to immunofluorescence methods with special focus to the
study of meiotic chromosomes in birds, mammals and some insects.