TRANSFERENCIA GENICA ASISTIDA POR CAMPOS MAGNETICOS: ESTUDIOS EN CELULAS GLIALES

JOAQUÍN PARDO, YOLANDA E. SOSA, PAULA CECILIA. REGGIANI, MAGDA LORENA ARCINIEGAS, FRANCISCO H SÁNCHEZ, RODOLFO G. GOYA

ACTA BIOQUíMICA CLíNICA LATINOAMERICANA

FEDERACION BIOQUIMICA PROVINCIA BUENOS AIRES

Lugar: La Plata; Año: 2013

0325-2957

It is known that certain types of magnetic nanoparticles (MNPs) complexed to gene vectors can,in the presence of an external magnetic field, greatly enhance gene transfer into cells. Thistechnique, called magnetofection, is of great relevance to gene therapy. In the present study theability of MNP/adenovector complexes to enhance gene transfer to B92 glial cells was assessed.Two complexes were assessed, namely PEI-Mag2/RAd-GFP and PEI-Mag2/RAd-DsRed,which are constituted by the MNP PEI-Mag2 complexed to the adenovector RAd-GFP(expressing the green fluorescent protein GFP) and RAd-DsRed (expressing the redfluorescent protein DsRed2), respectively. It was shown that for both vectors, anincrease in the ratio MNP/PVP (physical viral particle), is paralleled by an increase intransduction efficiency, up to a certain threshold value at which an efficiency plateau isreached. This threshold value was 0.5 fg Fe/PVP for the RAd-GFP complex and about71 fg Fe/PVP for the RAd-DsRed complex. We conclude that the two magneticcomplexes assessed in this study represent promising tools for enhancing the efficiencyof gene therapy in brain cells.