Evaluation of the angiogenic effects of novel nanostructured composites

HARO DURAND L, VARGAS GE, CADENA V, ROMERO M; VERA MESONES R, ZAGO MP, MACKOVIC M, SPALLEK S, SPIECKER E, BOCCACCINI AR, GORUSTOVICH A.

San Juan

Jornada; XIII Jornadas Anuales de la Sociedad Argentina de Biología. 2da Reunión Conjunta de las Sociedades de Biología de la República Argentina; 2011

Sociedades de Biología de la República Argentina

Publicado en Biocell 2011;35(3):A222    Collagen materials have been utilized in medicine because of their proven biocompatibility and capability of promoting wound healing. The aim of present study was to evaluate the effect of adding nanoscale silicate bioactive glass particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20-30 nm) BG particles of nominally 45S5 Bioglass® composition were used to prepare composite films, which were characterised by SEM-EDS and TEM. The angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an in-vivo model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing 41% the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces a strong angiogenic response making collagen/n-BG composites attractive matrices for applications in tissue engineering and regenerative medicine, in particular for the regeneration of highly vascularised tissues such as bone.