INIBIOMA   20415
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y MEDIOAMBIENTE
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Diversity of bacterial assemblages in ultraoligotrophic Andean lakes.
Autor/es:
ROBERTO BERTONI; CORNO, C.,; CARAVATI, E.; MODENUTTI, B.; BALSEIRO, E.; CRISTIANA CALLIERI
Lugar:
Parma Italia
Reunión:
Congreso; SITE 2008; 2008
Institución organizadora:
AIOL
Resumen:
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The North
Patagonian Andine lakes are unique environments with a simplified trophic chain
dominated by mixotrophic organisms. Up to now no data have been published on
the bacterial community inhabiting these ultraoligotrophic lakes. From one side
the presence of mixotrophic flagellates and ciliates supports the existence of
an important bacterial compartment. On the other hand, the high UV radiation
and the low temperature could create conditions for a slow growing bacterial
community. In austral summer 2006 we measured the bacterial activity with 14C Leucine uptake method for some of those lakes. A
very low activity (around 0.1 μgC l-1) indicated the existence of high limiting
conditions for bacterial growth. In 2008 we extended the study to other lakes
with the aim to measure the bacterial abundance and biovolume and to study the diversity
of bacterial assemblages using molecular approaches. The Denaturing Gradient
Gel Electrophoresis (DGGE) was used to assess the Operational Taxonomic Units
(OTU) diversity and a number of selected bands from DGGE gels were sequenced
for a deeper phylogenetic study. The results will be here discussed, evaluating
the relationship between the geographical location of the lakes (or their
physical connection) and the respective bacterial community composition.