INIBIOMA   20415
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y MEDIOAMBIENTE
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bacterial community diversity in ultraoligotrophic Andean lakes
Autor/es:
BERTONI, R.; CORNO, G.; CARAVATI, E.; MODENUTTI, B.E.; BALSEIRO, E.G; CALLIERI, C.
Lugar:
Parma, Italia
Reunión:
Congreso; SITE 2008; 2008
Institución organizadora:
AIOL
Resumen:
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The North Patagonian Andine
lakes are unique environments with a simplified trophic chain dominated by
mixotrophic organisms. Up to now no data have been published on the bacterial
community inhabiting these ultraoligotrophic lakes. From one side the presence
of mixotrophic flagellates and ciliates supports the existence of an important
bacterial compartment. On the other hand, the high UV radiation and the low temperature
could create conditions for a slow growing bacterial community. In austral
summer 2006 we measured the bacterial activity with 14C Leucine
uptake method for some of those lakes. A very low activity (around 0.1 μgC l-1) indicated the
existence of high limiting conditions for bacterial growth. In 2008 we extended
the study to other lakes with the aim to measure the bacterial abundance and
biovolume and to study the diversity of bacterial assemblages using molecular
approaches. The Denaturing Gradient Gel Electrophoresis (DGGE) was used to
assess the Operational Taxonomic Units (OTU) diversity and a number of selected
bands from DGGE gels were sequenced for a deeper phylogenetic study. The
results will be here discussed, evaluating the relationship between the
geographical location of the lakes (or their physical connection) and the
respective bacterial community composition.