Expression analysis of cytochrome P450 genes in pyrethroid-resistant Triatoma infestans

MORICONI DEBORA; SALAMANCA-MORENO JHON; CALDERÓN FERNÁNDEZ GUSTAVO M; DULBECCO ANDREA B; PEDRINI NICOLAS; JUÁREZ M PATRICIA

Congreso; LII Reunión de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

The blood-sucking bug Triatoma infestans (Hemiptera, Reduviidae) is the most widespread and relevant vector of Chagas disease in the southern region of South America. Although chemical insecticides have been a successful vector control strategy, several foci of resistance to deltamethrin have been recently documented. Among genes involved in detoxification processes in insects, cytochrome P450 monooxigenases (CYP) are often associated with relevant roles in metabolic resistance. Using the Rhodnius prolixus genome information available, we characterized 33 CYP genes belonging to clans 2, 3, 4, and mitochondrial in the integument of T. infestans. Five genes belonging to clan 3 and four genes from clan 4 were upregulated in deltamethrin-resistant fifth-instar nymphs compared with deltamethrin-susceptible specimen, as measured by qPCR. These results suggest that induction of specific CYP genes might be responsible, at least in part, for the high levels of deltamethrin resistance detected in T. infestans.