INVESTIGADORES
CASATI Paula
congresos y reuniones científicas
Título:
UV-B effect on DNA mismatch repair proteins
Autor/es:
LARIO L.; SPAMPINATO, CLAUDIA; PAULA CASATI
Lugar:
Campinas
Reunión:
Congreso; VIII Cong Aberto aos Estudantes de Biologia (CAEB); 2007
Institución organizadora:
CAEB
Resumen:
Plant DNA is continuously damaged by ultraviolet (UV)
irradiation. Plants generally have a higher tolerance for UV than
animals due to the absorption of UV by waxy substances on leaf
surfaces, cell walls and intracellular chemical compounds such as
flavonoids. However, UV radiation that is not absorbed induces
DNA damage that should be repaired to maintain genetic stability.
Different DNA repair mechanisms exist that are both specific and
overlapping. Mismatch repair (MMR) systems repair DNA
mismatches and participate in responses to a variety of adducts
induced by endogenous and exogenous agents. The MMR system
functions through the interactions between several proteins, such
as MSH2, MSH6, MLH1 and PMS1. Besides these proteins plants
also encode a unique MSH7. To investigate the regulation of plant
MMR by UV-B, we evaluated the transcripts levels of MMR
proteins by qRT-PCR. Our results demonstrate that MSH2, MSH6
and PMS1 mRNAs are induced by UV-B in A. thaliana leaves and
flowers, while MLH1 remained unchanged in these tissues. A
similar effect was observed for MSH2 and MSH6 from Z. mays.
Interestingly, MSH7 mRNA levels in controls plants of both
species were undetectable, while rapid induction was observed
after UV-B irradiation. The correlation with protein expression
and the effect of mutations in those genes in Arabidopsis using
SALK-lines are under progress.A. thaliana leaves and
flowers, while MLH1 remained unchanged in these tissues. A
similar effect was observed for MSH2 and MSH6 from Z. mays.
Interestingly, MSH7 mRNA levels in controls plants of both
species were undetectable, while rapid induction was observed
after UV-B irradiation. The correlation with protein expression
and the effect of mutations in those genes in Arabidopsis using
SALK-lines are under progress.Z. mays.
Interestingly, MSH7 mRNA levels in controls plants of both
species were undetectable, while rapid induction was observed
after UV-B irradiation. The correlation with protein expression
and the effect of mutations in those genes in Arabidopsis using
SALK-lines are under progress.