OXIDATIVE STRESS AND LIPOLYSIS: NEW INSIGHTS IN FAT METABOLISM

FUNK, M; MANISCALCHI, A; BENZI JUNCOS, O; ALZA, N; CONDE, M ; SALVADOR, G; URANGA, R

Mendoza

Congreso; . LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); 2022

Prolonged oxidative stress (OS) directly affects fat metabolism, with implications in the onset of obesity, insulin resistance, and type 2 diabetes. Particularly in adipocytes, it is well known that OS participates in several mechanisms related with proliferation and differentiation. Our aim was to study the signaling events underlying lipolysis triggered by OS. For this purpose, we worked with different adipocyte in vitro cultures (differentiated 3T3L1 and mesenchymal stem cells) and with an in vivo model, all subjected to iron-induced OS.3T3L1 adipocytes challenged with ferric ammonium citrate (FAC, 500-1000 µM) displayed augmented lipid peroxides and membrane permeability when compared with non-treated cells. The increase in OS markers observed in 3T3L1 adipocytes was coincident with a rise in glycerol release to the medium. These results were also corroborated in the in vivo model, where a decreased neutral lipid content in gonadal adipose tissue of iron-treated mice was observed. In addition, iron-treated animals presented a different architecture of gonadal fat characterized by cell shrinkage, decreased volume tissue, and fibrosis.Lipolysis in the white adipose tissue of humans and rodents is a step-wise process regulated by adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and monoacylglycerol lipase. Exacerbated lipolysis was accompanied by the upregulation of βcatenin expression in 3T3L1 and in gonadal adipocytes. To ascertain the role of this signaling pathway in OS-induced lipolysis, we worked with adipocytes differentiated from primary mesenchymal stem cells with wild type expression or deletion of β-catenin gene. To this end, stem cells were isolated from outer ears of β-catenin fl/fl mice and after differentiation to adipocytes, gene deletion was induced with adenoviral Cre recombinase. The expression of the lipolytic enzymes, ATGL and HSL, was evaluated by qRT-PCR in wild type and β-catenin knock out (β-catenin KO) adipocytes exposed to vehicle or FAC. In wild type adipocytes, iron exposure increased ATGL and HSL mRNA levels, whereas lipolytic enzyme expression remained unchanged in β-catenin KO cells. Our results demonstrate that iron-induced OS is able to activate lipolysis through a mechanism involving the β-cateninpathway in fat cells.