INVESTIGADORES
SALVADOR Gabriela Alejandra
artículos
Título:
Age-associated changes of Insulin action on the hydrolysis of diacylglycerol generated from phosphatidic acid
Autor/es:
SUSANA J, PASQUARE; SALVADOR, GABRIELA ALEJANDRA; MARIA GIUSTO, NORMA
Revista:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY
Editorial:
Elsevier
Referencias:
Año: 2006 p. 1 - 1
ISSN:
0010-406X
Resumen:
Age-related changes in insulin action on diacylglycerol (DAG) degradation was studied in rat cerebral cortex synaptosomes. The generation of
monoacylglycerol (MAG) and water soluble products (WSP, glycerol plus glycerol-3-phosphate) from DAG was studied in cerebral cortex (CC)
synaptosomes from adult (4-month-old) and aged (28-month-old) rats. Additionally, the effect of porcine insulin and tyrosine phosphorylation was
evaluated in the same group of animals. In this study we demonstrate that the age-related increase in WSP generation was accompanied by
unmodified MAG levels. In the presence of diacylglycerol lipase (DAG lipase) inhibitor, RHC-80267, a lower inhibitory effect on MAG
production was observed in CC synaptosomes from aged rats with respect to that in adult membranes. Under these experimental conditions, WSP
formation was only diminished in aged membranes. Insulin stimulated MAG and WSP formation at long incubation times (30 min) in adult
animals, while it had an inhibitory effect in aged animals. Insulin plus vanadate (as tyrosine-phosphatase inhibitor) inhibited MAG production at
short incubation times whereas the same effect was observed in aged animals at long times of incubation. WSP formation was stimulated by
insulin plus vanadate both in adult and aged animals at 30 min of incubation. Our results show that insulin differentially modulates MAG and
WSP production from exogenous PA in CC synaptosomes from aged rats compared with adult rats.
© 2006 Elsevier Inc. All rights reserved.