INVESTIGADORES
RUMBO Martin
artículos
Título:
Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
Autor/es:
DOMINIK MEIER; HERNÁN CAGNOLA; RAMISCH DIEGO; CAROLINA RUMBO; FERNANDO CHIRDO; GUILLERMO DOCENA; GONDOLESI GABRIEL; MARTIN RUMBO
Revista:
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 p. 138 - 145
ISSN:
0009-9104
Resumen:
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During
intestinal transplant (ITx) operation, intestinal lymphatics are not reconstituted.
Consequently, trafficking immune cells drain freely into the abdominal cavity.
Our aim was to evaluate whether leukocytes migrating from transplanted
intestine could be recovered from the abdominal draining fluid recovered though
peritoneal drainage in the early post-ITx period, and to determine potential
applications of the assessment of draining cellular populations. The cell
composition of the abdominal draining fluid was analyzed during the first 11
post-ITx days. By flow cytometry, immune cells from blood and draining fluid
samples obtained the same day showed an almost complete lymphopenia in
peripheral blood, whereas CD3+CD4+CD8-, CD3+CD8+CD4-,
and CD3-HLA-DR+CD19+ lymphocytes were the main
populations in the draining fluid. Non-complicated recipients evolved from a
mixed leukocyte pattern including granulocytes, monocytes, and lymphocytes to
an exclusively lymphocytic pattern along the first post-ITx week. At day 1-2
post-ITx, analysis by short tandem repeats fingerprinting of CD8+
sorted T cells from draining fluid indicated that 50% of cells were from graft
origin, whereas by day 11 post ITx, this proportion decreased to less than 1%.
Our
results show for the first time that the abdominal drainage fluid contains
mainly immune cells trafficking from the implanted intestine, giving the
opportunity to sample lymphocytes draining from the grafted organ along the
post-TX period. Therefore, this analysis may provide information useful for
understanding ITx immunobiology and eventually could also be of interest for
the clinical management.
