INVESTIGADORES
VERSTRAETEN Sandra Viviana
artículos
Título:
Procyanidins can interact with Caco-2 cell membrane lipid rafts: involvement of cholesterol.
Autor/es:
VERSTRAETEN SV; JAGGERS GK; FRAGA CG; OTEIZA PI
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 1828 p. 2646 - 2653
ISSN:
0005-2736
Resumen:
Large procyanidins (more than three subunits) are not absorbed at the
gastrointestinal tract but could exert local effects through their
interactions with membranes. We previously showed that hexameric
procyanidins (Hex), although not entering cells, interact with membranes
modulating cell signaling and fate. This paper investigated if Hex, as
an example of large procyanidins, can selectively interact with lipid
rafts which could in part explain its biological actions. This mechanism
was studied in both synthetic membranes (liposomes) and Caco-2 cells.
Hex promoted Caco-2 cell membrane rigidification and dehydration,
effects that were abolished upon cholesterol depletion with
methyl-β-cyclodextrin (MCD). Hex prevented lipid raft structure
disruption induced by cholesterol depletion/redistribution by MCD or
sodium deoxycholate.
Supporting the involvement of cholesterolHex bonding in Hex
interaction with lipid rafts, the absence of cholesterol markedly
decreased the capacity of Hex to prevent deoxycholate-
and Triton X-100-mediated disruption of lipid raft-like liposomes.
Stressing the functional relevance of this interaction, Hex mitigated
lipid raft-associated activation of the extracellular signal-regulated
kinases (ERK) 1/2. Results support the capacity of a large procyanidin
(Hex) to interact with membrane lipid rafts mainly through
Hexcholesterol bondings. Procyanidinlipid raft interactions can in
part explain the capacity of large procyanidins to modulate cell
physiology.