An early treatment with 17-ß-estradiol is neuroprotective against the long-term effects of neonatal ionizing radiation exposure

CACERES, L.G.; URAN S; ZORRILLA ZUBILETE; ROMERO JI; CAPANI F; GUELMAN L

JOURNAL OF NEUROCHEMISTRY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2011

0022-3042

Abstract Ionizing radiations can induce oxidative stress on target tissues, acting mainly through reactive oxygen species (ROS). The aim of this work was to investigate if 17-b-estradiol (bE) was able to prevent hippocampal-related behavioral and biochemical changes induced by neonatal ionizing radiation exposure and to elucidate a potential neuroprotective mechanism. Male Wistar rats were irradiated with 5 Gy of X-rays between 24 and 48 h after birth. A subset of rats was subcutaneously administered with successive injections of bE or 17-a-estradiol (aE), prior and after irradiation. Rats were subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. 17-a-estradiol (aE), prior and after irradiation. Rats were subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was able to prevent hippocampal-related behavioral and biochemical changes induced by neonatal ionizing radiation exposure and to elucidate a potential neuroprotective mechanism. Male Wistar rats were irradiated with 5 Gy of X-rays between 24 and 48 h after birth. A subset of rats was subcutaneously administered with successive injections of bE or 17-a-estradiol (aE), prior and after irradiation. Rats were subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. 17-a-estradiol (aE), prior and after irradiation. Rats were subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. b-estradiol (bE) was able to prevent hippocampal-related behavioral and biochemical changes induced by neonatal ionizing radiation exposure and to elucidate a potential neuroprotective mechanism. Male Wistar rats were irradiated with 5 Gy of X-rays between 24 and 48 h after birth. A subset of rats was subcutaneously administered with successive injections of bE or 17-a-estradiol (aE), prior and after irradiation. Rats were subjected to different behavioral tasks to evaluate habituation and associative memory as well as anxiety levels. Hippocampal ROS levels and protein kinase C (PKC) activity were also assessed. Results show that although bE was unable to prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in bE-treated irradiated rats approached control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. control values. In addition, aE administered to irradiated animals was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. was effective in preventing radiation-induced alterations. In conclusion, bE was able to counteract behavioral and biochemical changes induced in irradiated animals, probably acting through an antioxidant mechanism. changes induced in irradiated animals, probably acting through an antioxidant mechanism. prevent radiation-induced hippocampal PKC activity changes, most behavioral abnormalities were reversed. Moreover, hippocampal ROS levels in b