Cytoprotective Effect of Valeriana officinalis Extract on an In Vitro Experimental Model of Parkinson Disease

DE OLIVERIA DM, BARRETO G, DE ANDRADE DV, SARACENO E, AON-BERTOLINO L, CAPANI F, DOS SANTOS EL BACHÁ R, GIRALDEZ LD.

NEUROCHEMICAL RESEARCH

SPRINGER/PLENUM PUBLISHERS

Año: 2008 p. 234 - 241

0364-3190

Parkinson0s disease (PD) is one of the most important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of 0s disease (PD) is one of the most important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of Valeriana officinalis on rotenone-induced apoptosis in  human neuroblastoma SH-SY5Y cells were demonstrated. The cytotoxicity, cell viability and analysis of cellular morphology were performed by MTT-tetrazole (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and phase contrast microscopy, respectively. Significant changes in the cellular morphology, and condensation of the cell body could be observed when cells were treated with 300 nM rotenone for 48 h. Three different concentrations of Valeriana officinalis extract were  used (0.049, 0.098 and 0.195 mg/mL). These extracts brought about an increase of 7.0 ± 1.3%, 14.5 ± 1.3% and  14.5 ± 3.2% in cell viability. Our results indicated that  neuroprotector action of the Valeriana officinalis extract  provides support for later studies as they help understanding this drug for the development of  cytoprotective various therapies in PD.0s disease (PD) is one of the most important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of Valeriana officinalis on rotenone-induced apoptosis in  human neuroblastoma SH-SY5Y cells were demonstrated. The cytotoxicity, cell viability and analysis of cellular morphology were performed by MTT-tetrazole (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and phase contrast microscopy, respectively. Significant changes in the cellular morphology, and condensation of the cell body could be observed when cells were treated with 300 nM rotenone for 48 h. Three different concentrations of Valeriana officinalis extract were  used (0.049, 0.098 and 0.195 mg/mL). These extracts brought about an increase of 7.0 ± 1.3%, 14.5 ± 1.3% and  14.5 ± 3.2% in cell viability. Our results indicated that  neuroprotector action of the Valeriana officinalis extract  provides support for later studies as they help understanding this drug for the development of  cytoprotective various therapies in PD.