DYNAMICS OF SOLUBLE IMMUNE MEDIATORS IN COVID-19 PATIENTS FROM AN ARGENTINEAN COHORT WITH MODERATE AND SEVERE SYMPTOMS

LAURA ALMADA; OTROS (VER EN RESUMEN); G MORON; GRUPO INMUNOCOVIDCBA

virtual

Congreso; LXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2021

SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)

Laura Almada*, Sofia C Angiolini*, Nicolás Dho N*, JeremiasDutto J*, Yamila Gazzoni Y*, Clarisa Manzone C*, ConstanzaMarín *, Nicolás E Ponce, Pablo Iribarren, Fabio Cerbán,Gabriel Morón, Carolina Amezcua Vesely, Yamile Ana, LauraCervi, Laura Chiapello, Laura Fozzatti L, Paula Icely, MarianaMaccioni, Cristian Mena, Carolina Montes, Cristina Motrán ,Cecilia Rodríguez Galán, Cinthia Stempin, María EstefaniaViano, M Bertone#, Claudio Abiega#, Daiana Escudero#,Adrian Kahn#, Juan Pablo Caeiro#, Daniela Arroyo, BelkysMaletto , Eva Acosta Rodríguez, Adriana Gruppi, Claudia ESotomayor. Grupo ImmunoCovidCBA.*All these authors contributed equally.Departamento de Bioquímica Clínica, CIBICI-CONICET, Facultadde Ciencias Químicas, Universidad Nacional de Córdoba.#Hospital Privado Universitario de Córdoba, IUCBC.The cytokine storm, a form of systemic inflammatory response syndrome,is one of the most dreadful complications that can occurduring COVID-19. In this work, we aimed at studying the occurrenceof a cytokine storm in a cohort of COVID-19 patients (Cpts) from Córdoba(Argentine). During first wave, we collected sera from individualswith RT-PCR+ for SARS-CoV2 hospitalized in Hospital Privadowith moderate (MOD) and severe (SEV) disease (n=62, aged 21-80years) as well as healthy controls (HC n=24, age matched), to determinethe concentrations of IL-1b, IL-6, IL-8, IL-10, IL-12, IL-28A/B,IL-29, TNF, IP-10, IFNa2, IFNb, IFNg and GM-CSF by LEGENDplexTM.Males represented 74% of MOD and 67% of SEV Cpts. Hypertension(HT, 48%), obesity (31%), dyslipidemia (DL, 24%), anddiabetes (24%) were the most frequent comorbidities. All cytokines,except IL-28A/B, were significantly increased in total Cpts in comparisonwith HC (p<0.01). Elevated levels of IL-6 and CRP (p<0.01)between 4-7 day after hospitalization were found in all Cpts whodied, but the cytokine profiles were different in deceased SEV thanin MOD Cpts. Mortality in SEV group was associated with high levelsof IL-6 (p<0.001), GM-CSF (p<0.01), IL-8, CRP, leukocytosis anddecreased platelets (p<0.0001 for all). Comorbidities were linked toparticular patterns of immune mediators on admission but not afterwardsduring infection, with HT Cpts exhibiting increased IP-10 levelsbut DL Cpts showing lower concentration of IL-1b, GM-CSF and IL-10 compared to no-HT and no-DL Cpts, respectively (p<0.05).The frequency of Cpts who required O2 support was higher in HT(84%) vs DL (67%). Although our data have similarities with thosein international reports, the complete profiling of different parameters(cytokine/chemokines, risk factors, epidemiological and clinicalcharacteristics) in the local cases add value by identifying particularitiesthat may be relevant for the management and prognosis duringSARS-CoV2 infection.