) ALTERATIONS IN VASCULAR INTEGRITY AND NEURONAL FUNCTIONALITY RELATED TO EARLY STAGES OF DIABETIC RETINOPATHY IN A METABOLIC SYNDROME MOUSE MODEL

PAZ MC; BARCELONA PF; SUBIRADA CALDARONE PAULA; RIDANO MAGALI E; CASTRO C; CHIABRANDO GA; SANCHEZ MC

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Alterations in vascular integrity and neuronal functionality related to early stages of diabetic retinopathy in a metabolic syndrome mouse model.Paz, MC; Barcelona, PF; Subirada, PV; Ridano, ME; Castro, C; Sánchez, MC.Diabetic retinopathy (DR) is the most serious ocular complication associated with Type 2 Diabetes Mellitus (T2DM), which is a metabolic syndrome (MS), and one of the leading causes of blindness. Thus, we proposed to analyze in a MS mouse model, markers of retinal vascular integrity and neuronal funcionality, related to early stages of DR.We used C57BL/6 (WT) and Apolipoprotein E knockout (ApoE-KO) mice either fed with a normal diet (ND) or a 10% w/v fructose diet (FD) in drinking water from 2 months of age. We demonstrated that ApoE-KO after 2 month of FD, presented hypercolesterolemia, hypertriglyceridemia, hyperglycemia and hyperinsulinemia. Here, retinal functionality was assessed by scotopic ERG at 4 month of FD treatment. Extravasation of serum proteins and levels of proteins involved in neuro-glial injury were analyzed by WB. Vascular permeability was evaluated by albumin?Evans blue complex leakage and astrocyte GFAP levels on whole mounts of retina.The ERG a-wave and the OPs amplitudes were significantly decrease in retinas of ApoE-KO after 4 month of FD vs. WT DN (p