The CRE-binding factor ATF-2 facilitates the occupation of the CCAAT box in the fibronectin gene promoter

SREBROW A; MURO AF; WERBAJH SE; SHARP PA; KORNBLIHTT AR

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1993 vol. 327 p. 25 - 28

0014-5793

@font-face { font-family: "ＭＳ 明朝"; }@font-face { font-family: "Cambria Math"; }@font-face { font-family: "Cambria"; }p.MsoNormal, li.MsoNormal, div.MsoNormal { margin: 0cm 0cm 0.0001pt; font-size: 12pt; font-family: Cambria; }.MsoChpDefault { font-family: Cambria; }div.WordSection1 { page: WordSection1; } The cAMP response element (CRE) and the CCAAT box of the fibronectin gene promoter are separated by only twenty base pairs. A specific factor that binds the CRE interacts cooperatively with the protein which binds to the adjacent CCAAT box, stimulating transcription [1992, J. Biol. Chem. 267, 12767-12774]. Here we show that the CRE factor is an heterodimer between a 43 kDa and the 73 kDa CRE-binding proteins and we identify the latter as ATF-2 (also named CRE-BPI), a protein implicated in recruiting transcriptional activators to promoters, able to form heterodimers with Jun and for which a sequence-deduced MW of 55 kDa had been previously reported.