INVESTIGADORES
MIRANDA Maria Victoria
artículos
Título:
Partition behaviour of thaumatin derivatives in poly(etlyleneglycol)/salt aqueous two-phase systems
Autor/es:
MIRANDA, MARÍA VICTORIA; FERNÁNDEZ LAHORE, HÉCTOR MARCELO; OSVALDO CASCONE,
Revista:
BIOSEPARATION
Referencias:
Año: 1996 vol. 6 p. 315 - 321
ISSN:
0923-179X
Resumen:
In order to assess üe influence of the protein charge on its partitioning behaviour in poly(ethyleneglycol/salt
aqueous two-phase systems, four thaumatin derivatives with isoelectric points around 4.0, 5.2,8.7 utd 9.2 were
obtained by succinylation and isolated by isoelectric focusing and chromatofocusing. The modification reaction
was mild enough to preserve the tertiary structure of the proteins, as judged by circular dichroism measurements
and fourth derivative UV specha. The surface hydrophobicity of the thaumatin derivatives with pls 5.2,8.7 aÍd9.2
- as determinedb y hydrophobici nteractionc hromatography- was not modified by the succinylationt reatment.
Partition constantso f the derivativesi n poly(ethyleneglycol)/phosphatec,i trate and sulphatea queoust wo-phase
systems at pHs between 7.0 and 9.5 indicate that the partition is not dependent on the protein charge in the
poly(ethyleneglycol)/salts ystemss tudied.