Sulfanilic acid-modified chitosan mini-spheres and their application for lysozyme purification from egg white

DANIELA HIRSCH; BAIELI FERNANDA; NICOLÀS URTASUN; LAZARO MARTINEZ; ROMINA GLISONI; MARIA V MIRANDA; CASCONE O; WOLMAN FEDERICO

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2018

8756-7938

A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographicmatrix was physico-chemically characterized by ss-NMR and f potential, and itschromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 6 1.47 mg g21 while the dissociation constant was 0.074 6 0.012 mg mL21. The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP-HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials.