Partition behaviour of horseradish peroxidase isoenzynes in aqueous two-phase poly(ethyleneglycol)/phosphate systems.

MIRANDA, MARÍA VICTORIA; OSVALDO CASCONE,

BIOTECHNOLOGY TECHNIQUES

Año: 1994 vol. 8 p. 275 - 280

0951-208X

Partition of purified horseradsih peroxidase isoenzymes in aqueous two-phase systems was not affected by pH changes, but tie-line length and NaCl addition greatly increased the partition coefficient of all three isoenzymes. The former having more influence than the latter. In all systems, K were higher for acidic than those for neutral and asic isoenzymes, and K for basic were the lowest.