Neutrophil autophagy duriing human active tuberculosis is modulated by SLAMF1.

PELLEGRINI JM; SABBIONE F; MORELLI MP; MARTÍN C; TATEOSIAN NL; CASTELLO FA; AMIANO NO; PALMERO D; LEVI A; CIALLELA L; COLOMBO MI; TREVANI AS; GARCIA VE

San Miguel de Tucumán

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología.; 2019

BACKGROUND: Neutrophils infected with Mycobacterium tuberculosis (Mtb) predominate inpatient´s lungs. Neutrophilic infiltration represents a characteristic feature of severetuberculosis (TB) but neutrophil biology during TB remains poorly understood. Autophagy iscrucial for several neutrophil functions and can be modulated by different mediators. Thecostimulator SLAMF1 acts as a microbial sensor in macrophages but its expression in humanneutrophils has not been evaluated.METHODS: We investigated whether SLAMF1 participates in neutrophil autophagy against Mtb.Neutrophils were isolated from heparinized blood from TB patients and healthy donors andcultured (2x106 cells/ml) with Mtb-Ag (10μg/ml) ± SLAMF1 mAb (10μg/ml) during 2 h. Someexperiments were performed in the presence of a NADPH-oxidase inhibitor. SLAMF1 andautophagy levels were analyzed by flow cytometry and confocal microscopy. P values of