Deleterious effects of activated neutrophils and nets on trophoblast cell function in a model of maternal fetal interaction

CALO G; SABBIONE F; PASCUALI N; KEITELMAN I; VOTA D; PAPARINI D; RAMHORST R; PARBORELL F; TREVANI, AS; PÉREZ LEIRÓS, CLAUDIA

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología. Mar del Plata 14-17 de noviembre, 2018, Argentina.; 2018

Sociedad Argentina de Inmunología

Trophoblast cells (Tb) interact with different maternal immune cellpopulations at early pregnancy promoting an anti-inflammatory andtolerogenic response. Uterine vasculature remodeling occurs to accommodate the increasing demand of the growing fetus. Circulatingneutrophils are found activated during pregnancy and even moreduring pathological pregnancies. Vasoactive Intestinal Peptide (VIP)is a pleiotropic peptide with immunomodulatory effects through itsaction on VPAC1 and VPAC2 receptors, both found in neutrophils.We have already shown that VIP and conditioned media (CM) fromhuman first trimester Tb (Swan-71 cell line) inhibit PMA-inducedneutrophil extracellular trap(NET) formation, promote neutrophilapoptosis and revert the anti-apoptotic effect of LPS.Our aim was to evaluate the effect of VIP and trophoblast-derivedfactors on neutrophils and how these conditioned cells could impactlikewise on Tb function and profile.Neutrophils were isolated from healthy donors blood. We found thatboth neutrophils and monosodium urate crystals (MSU)-inducedNETs increased the expression of trophoblast IL-8 and decreasedTGF-β explored by RT-qPCR (P