Gamma Delta T lymphocytes modulate neutrophils function

CHIAPETTA C; SHIROMIZU, CAROLINA MAIUMI; ROSSO D; TOWSTYKA N; KEITELMAN I; SABBIONE F; TREVANI, AS; JANCIC C

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología. Mar del Plata 14-17 de noviembre, 2018, Argentina.; 2018

Sociedad Argentina de Inmunología

We have previously reported that neutrophils acquired differentprofiles of activation when they are cultured in presence of γδ Tcells activated by phosphoantigens or through CD3. Now, we aimto investigate the mechanism involved in the modulation of neutrophils activity by γδ T lymphocytes. γδ T cells were purified from human peripheral blood mononuclear cells by using an anti-TCR γδMicroBead isolation kit; and neutrophils were isolated by dextransedimentation. After purification, γδ T cells were stimulated with anti-CD3 antibodies (250 ng/ml) or phosphoantigens (HMBPP, 10 µM)and cultured with or without neutrophils. After that, we analyzed inγδ T cells, IFN-γ and TNF-α production by ELISA, and CD69 expression by flow cytometry. Also, we analyzed the activation state ofneutrophils and the expression of perforin by flow cytometry. Treatment of γδ T cells with HMBPP or anti-CD3 antibodies increasedthe expression of CD69 and the production of IFN-γ and TNF-α(p