INVOLVEMENT OF NEUTROPHIL EXTRACELLULAR TRAPS IN HEMOLYTIC UREMIC SYNDROME

RAMOS V; MEJÍAS P; SABBIONE F; ABREY-RECALDE MJ; FERNANDEZ BRANDO R; TREVANI AS; PALERMO M

Los Cocos, Córdoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

Hemolytic Uremic Syndrome (HUS), a vascular disease characterized by hemolityc anemia, thrombocitopenia and acute renal failure is caused by enterohemorraghic Shiga toxins(Stx)-producing bacteria. Besides Stx, inflammatory response mediated by neutrophils (PMN) is essential to HUS evolution. The aim of this work was to determine the Stx direct effect on PMN and the possible induction of neutrophil extracellular traps (NETs). For this purpose PMN were purified from peripheral blood of healthy donors. Then they were incubated with Stx and the surface expression of activation markers such as CD11b and CD66b was evaluated by flow cytometry. Both markers were increased following stimulation. (MFI±SE: CD66b: basal=65±11, Stx=154±11; CD11b: basal=471±48, Stx=619±100). We also evaluated the capacity of Stx to induce PMN the extrusion of NETs. Isolated PMN were incubated with phorbol myristate acetate (PMA), a positive inductor of netosis, or Stx and cells stained with propidium iodide and anti-elastase antibody and cells that underwent NETosis were counted by confocal microscopy (Basal=0, PMA=5.6*± 1, Stx=2.8±0.24*; n=3, *p