The blockade of low affinity neurotensin receptor alters mitochondrial bioenergetics and the triade NMDA receptor, PSD-95 and nNOS proteins.

LORES ARNAIZ, S.; KARADAYIAN, A.G.; GUTNISKY, A.; MIRANDA, J.; RODRÍGUEZ DE LORES ARNAIZ, G.

Villa Carlos Paz, Córdoba

Congreso; XXXIV Reunión Anual SAN 2019; 2019

Sociedad Argentina de Neurociencias

In previous work we have shown the impairment of nitric oxide (NO) production and mitochondrial alterations after the blockade of low affinity neurotensin receptor (NTS2) by levocabastine. A functional interaction between neuronal nitric oxide synthase (nNOS) and NMDA receptor through assembly with PSD-95 protein has been described. Male Wistar rats were i.p. injected with levocabastine (50 μg/kg) or saline and sacrificed 18 hours later. Brain cortex crude mitochondrial fractions and synaptosomal membranes were isolated by differential and sucrose gradient centrifugation, respectively. Levocabastine administration decreased 31% and 34% state 3 respiratory rates assayed with malate-glutamate and succinate, respectively, and 21% the mitochondrial membrane potential (p