Alterations in mitochondrial bioenergetics and nitric oxide metabolism due to acute alcohol exposure in mouse brain cortex synaptosomes and non-synaptic mitochondria.

KARADAYIAN, A.G.;; CZERNICZYNIEC, A.; ; LOMBARDI, P.; ; BUSTAMANTE, J.; ; LORES ARNAIZ, S.

Mar del Plata

Congreso; Reunión Anual SAIC - SAI - SAFE 2016; 2016

Sociedad Argentina de Investigación Clínica

The abuse of alcohol consumption is a type of alcohol-related disorder causing a number of serious consequences to health. Recently, we demonstrated that alcohol hangover (AH) induced a strong oxidative stress in brain cortex. Specifically, synaptosomes (S) resulted to be more affected due to AH than non-synaptic mitochondria (NSm). The aim of the present work was to study mitochondrial bioenergetics and NO metabolism at the beginning of AH in brain cortex NSm and S. Swiss male mice were treated with a single i.p. injection of saline (control group) or ethanol (3.8 g/kg; AH group). Biochemical assays were performed 6 h post-treatment when blood alcohol concentration was close to zero (AH start). Mitochondrial respiration, respiratory complexes activity, mitochondrial transmembrane potential, UCP-2 expression were determined. Also, nNOS expression, NOS activity and NO levels were assayed. Results showed a significant decrease in respiratory function in both subcellular fractions (p