INVESTIGADORES
LORES ARNAIZ Silvia
congresos y reuniones científicas
Título:
Protective Effects of the Synthetic Cannabinoids CP55,940 and JWH-015 on Rat Brain Mitochondria upon Paraquat Exposure.
Autor/es:
BUSTAMANTE, J.; VELEZ-PARDO, C.; JIMENEZ-DEL RIO, M.; LORES ARNAIZ; S.
Lugar:
Santiago de Chile
Reunión:
Congreso; Free Radicals and Antioxidants in Chile, VI Meeting of the SFRBM South American Group; 2009
Institución organizadora:
SFRBM South American Group
Resumen:
The
effect of cannabinoids CP55,940 and JWH-015 in rat brain mitochondria
after acute paraquat (PQ) exposure was analysed by their ability to
scavenge superoxide anion radicals and hydrogen peroxide in either sub-mitochondrial particles or intact mitochondria treated with PQ. Oxygen consumption and mitochondrial membrane potential (DYm)
were determined as parameters of mitochondrial function in the presence
of PQ alone or in combination with the cannabinoid agonists. Both
cannabinoids effectively attenuate mitochondrial damage against
PQ-induced oxidative stress by scavenging superoxide anion radical and
hydrogen peroxide. While H2O2 generation increased almost 3-fold upon PQ treatment (p<0.01) compared to untreated mitochondria, CP55,940 and JWH-015 significantly reduced mitochondrial H2O2 by 44% and 50%, respectively when co-incubated with PQ. Both cannabinoids
were not able to rescue mitochondrial respiration from PQ treatment,
effects on state 4 and 3 respiration, will be discussed. Mitochondria
incubated with 250 mM PQ, show moderate loss of basal Dym by about 22% compared to untreated mitochondria. Interestingly, both CP55,940 and JWH-015 were able to return Dym to basal state levels when co-incubated with PQ. In summary, CP55,940 and JWH-015 protect mitochondria against PQ-induced oxidative stress by maintaining Dym and efficiently scavenging O2.- and H2O2.
Understanding the mechanistic action of cannabinoids on mitochondria
might provide insights into more effective therapeutic approaches for
Parkinsons disease.