Acetaldehyde-Mediated Mitochondrial Dysfunction in Alcohol Hangover.

KARADAYIAN, A., ; CZERNICZYNIEC, A.; CARRERE, L.; GUERRA, J.I.; LORES ARNAIZ, S.

Buenos Aires

Simposio; Frontiers in Bioscience Symposium 4th edition.; 2023

Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), Partner Institute of the Max Planck Society

Alcohol hangover (AH) is characterized by physical and mental symptoms experienced after heavy drinking, occurring when blood alcohol concentration (BAC) approaches zero. In this study, we investigated the role of acetaldehyde in the deleterious effects of AH on mitochondrial function at brain cortex synapses. Using 4-methylpyrazole (4MP), an alcohol dehydrogenase (ADH) inhibitor, we aimed to elucidate the impact of acetaldehyde on AH-induced mitochondrial dysfunction.Male Swiss mice were divided into four groups: saline (control), 4MP (10 mg/kg), ethanol (3.8 g/kg, AH group), and 4MP-ethanol. Animals were sacrificed after 6 hours (BAC=0), and brain cortex synaptosomes were analyzed. We found that reducing acetaldehyde levels significantly restored mitochondrial respiration, ATP synthesis, and coupling efficiency, exhibiting a five-fold enhancement compared to the AH group (p