INVESTIGADORES
LORES ARNAIZ Silvia
congresos y reuniones científicas
Título:
Impact of hyperbaric atmosphere on brain mitocondrial function.
Autor/es:
LA PADULA, P.; KARADAYIAN, A., ; LORES ARNAIZ, S., ; DI GIROLAMO, G., ; COSTA, L.E., ; CZERNICZYNIEC, A.
Lugar:
Mar del Plata
Reunión:
Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2022
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Chronic hyperbaric treatment is often used to hasten tissue recovery and improve the physiological aspects of the damaged tissue. Taking into account that the brain is an organ highly sensitive to oxygen levels and possesses the highest energy demand, we evaluated the mitochondrial functionality and reactive oxygen species (ROS) production in cerebral cortex and hippocampus using an animal model of hyperbaria. Male Sprague Dawley rats were subjected to 30 sessions of 60 min in a hyperbaric chamber at 1.44 atm. and 100% O2. Mitochondrial oxygen consumption, respiratory efficiency (ADP/O) and nitric oxide (NO) levels were preserved in the cerebral cortex after chronic hyperbaric treatment. In addition, a 31% increase in superoxide anion production and a decrease in blood supply were observed. Regarding hippocampus, hyperbaric conditions decreased mitochondrial oxygen consumption (16% and 20 % for state 3 and 4, respectively) with the consequent reduction in superoxide anion levels (38%) and an inhibition of NO production (32%), while preserving respiratory efficiency (ADP/O). Conclusions: after a stimulus in the variation of the oxygen pressure in the inspired air, the hippocampus displays physiological adaptations at the mitochondrial level which lead to a decrease in the production of ROS. While the cerebral cortex, a more robust tissue, maintains its respiratory function assuming the impact of the increase in ROS. These results are in agreement with our previous studies of the effect of low oxygen pressure in the inspired air, where the mitochondrial functionality in cerebral cortex was preserved and the hippocampal mitochondria displayed different strategies to adapt to different oxygen pressures.
