Dopamine modifies oxygen consumption and mitochondrial membrane potential in striatal mitocondria.

CZERNICZYNIEC, A.; BUSTAMANTE, J.; LORES-ARNAIZ, S.

MOLECULAR AND CELLULAR BIOCHEMISTRY

SPRINGER

Año: 2010 vol. 341 p. 251 - 257

0300-8177

Dopamine is a neurotransmitter that has been related to mitochondrial dysfunction. In the present study, striatal intact mitochondria and submitochondrial membranes were incubated with different dopamine concentrations and changes on mitochondrial function, on hydrogen peroxide and nitric oxide production were evaluated. A 35% decrease in state 3 oxygen uptake (active respiration state) was found after 1 mM dopamine incubation. In addition, mitochondrial respiratory control significantly decreased, indicating mitochondrial dysfunction. High dopamine concentrations induced mitochondrial depolarization. Also, evaluation of hydrogen peroxide production by intact striatal mitochondria showed a significant increase after 0.5 and 1 mM dopamine incubation. Incubation with 0.5 and 1 mM dopamine increased nitric oxide production in submitochondrial membranes by 28% and 49% respectively as compared with control values. This work provides evidence that high dopamine concentrations induce striatal mitochondrial dysfunction through a decrease in mitochondrial respiratory control and loss of membrane potential, probably mediated by free radical production.