Ketamine effect on intracellular and mitochondrial calcium mobilization.

BUSTAMANTE, J.; CZERNICZYNIEC, A. ; LORES-ARNAIZ, S

BIOCELL

Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo/CONICET

Lugar: Mendoza; Año: 2016 vol. 40 p. 11 - 14

The suppressive effect of ketamine on intracellular calcium has been reported in a variety of cells although the mechanisms involved are not well understood. The aim of this work was to evaluate the effect of ketamine on mitochondrial Ca2+ accumulation and on cellular Ca2+ mobilization using FLUO4-AM and flow cytometry. The results showed that hippocampal mitochondria from ketamine injected animals presented a lower ability to retain calcium at concentrations higher than 20 M, as compared with controls (saline injected animals). In addition, ketamine produced a significant decrease in KCl-induced intracellular Ca2+ influx in Vero cell cultures. According to these data, ketamine produces a clear inhibitory effect on cytosolic Ca2+ transport mechanisms, independent of the Ca2+ channel associated NMDA receptor.