Preliminary assessment of three plant extracts to obtain silver nanoparticles for their application as biocide for coatings

BARBERIA-ROQUE, L.; GAMES ESPINOSA E.; VIERA, M.; BELLOTTI, N.

Manchester

Simposio; XVII International Biodegradation & Biodeterioration Symposium; 2017

International Biodeterioration and Biodegradation Society

In the last decades, the interest in the study of the biodeterioration in buildings has increased due to the consequences that it generates in indoor environment and human health. The control of biodeterioration has led to the development of new biocides for the application in coating formulations among which are the nanoparticles. Green synthesis of nanoparticles based on the use of aqueous plant extracts is a promising technique as it is accessible, effective, low cost and eco-friendly. The aim of this research was to carry out a preliminary assessment of the use of aqueous extracts from three native argentine plants: Ilex paraguariensis Saint Hilaire, Schinus molle and Equisetum giganteum for the synthesis of silver nanoparticles (AgNp).The plant extracts obtained with Mili-Q water at 80°C were used to synthesized NpAg with AgNO3 solutions, 10-1M and 10-2M at pH = 7 and 60°C. UV-vis spectroscopy was carry. The evaluation of the antimicrobial activity of the products selected against: Escherichia coli (ATCC 6538), Staphylococcus aureus (ATCC 11229), Chaetomium globosum (KU936228) and Alternaria alternata (KU936229).were performed using the agar diffusion technique (Kirby-Bauer) The solutions obtained with the lower AgNO3 solution presented spectra with bands of absorption with maxima ~ 450 nm which confirm the presence of NpAg. The bands differed in width which is related to a wider particle size distribution, being AgNp from Ilex paraguariensis the one with the widest and highest absortion intensity. The diffusion test showed that AgNps were active in every case against both fungi and bacteria.