Vesicular trafficking of NMDA like receptors in Neohelice granulata (Chasmagnathus granulatus)

Y HEPP; FREUDENTHAL RAMIRO; ME PEDREIRA

Huerta Grande

Congreso; XXVI Congreso Anual SAN; 2011

SAN

Vesicular trafficking of NMDA like receptors in Neohelice granulata (Chasmagnathus granulatus) The NMDA glutamate receptors (NMDARs) mediate the majority of excitatory neurotransmission in the nervous system. Molecular and electrophysiological properties suggest that they may be the Hebbian´ coincidence detectors and has been implicated in synaptic plasticity, learning and memory. In the crab Neohelice granulata was demonstrated that the systemic administration of NMDAR antagonists (MK-801, APV) affects the memory consolidation, reconsolidation and extinction memory of the Context-Signal Memory. These results indicate that NMDA like receptors have been involved in long-term memory in this invertebrate model. Here we characterized the NMDA like receptors by Western blot assays and immunohistochemistry techniques. The next step was to evaluate the NR1 like receptor subunit expression as consequence of learning. Additionally, differential surface expression of NR1 like subunit was studied by semi-quantification of the intracellular and extracellular vesicular pool.