ADXR-ADX-P450, a putative electron transport system in plant mitochondria.

DISTEFANO A; BELLIDO A; FIOL D; EDUARDO JULIAN ZABALETA; PAGNUSSAT G

Rosario

Conferencia; 8th Internationl Conference of Plant Mitochondrial Biology; 2013

ICPMB organizing team

In mammals and insects ADX mediates electron transfer from NADPH via ADRX to the terminal acceptor, mitochondrial P450 which constitutes the fiorst step in steroid hormone synthesis. A thaliana has an ADRX homologue and 2 ADX which are also located in the mitochondria. Alhotugh there 244 P450s in Arabidopsis, no known P450 located in mitochondria. To study the possibility that a similar biosynthetic hormone pathway exists in plants we analyzes putative P450 proteins that may be located in plant mitochondria via Gfp fusion proteins and transient transformation of N bethamiana. An independent approach used yeast two hybrid system. T- DNA insertional mutants were analysed showing defect in gametophyte development.