A NOVEL -CARBONIC ANDHYDRASE FROM Arabidopsis thaliana INVOLVED IN RETROGRADE REGULATION

VILLARREAL, F.; MARTIN, M. V.; EDUARDO JULIAN ZABALETA

Pinamar

Congreso; PABMB 10th congress; 2005

A novel group of 5 ã-Carbonic anhydrases (AtãCAs)has been recently identified in Arabidopsis thaliana. These proteins are targeted to mitochondria, and they are specifically located bounded mainly to the Complex I (NADH-ubiquinone oxidorreductase) of the mitochondrial electron transport chain. Proteomic studies ofã-Carbonic anhydrases (AtãCAs)has been recently identified in Arabidopsis thaliana. These proteins are targeted to mitochondria, and they are specifically located bounded mainly to the Complex I (NADH-ubiquinone oxidorreductase) of the mitochondrial electron transport chain. Proteomic studies ofArabidopsis thaliana. These proteins are targeted to mitochondria, and they are specifically located bounded mainly to the Complex I (NADH-ubiquinone oxidorreductase) of the mitochondrial electron transport chain. Proteomic studies of atãca2 and atãca3 plants revealed that complex I levels are diminished about 80% and 10%, respectively. Cell cultures show lower growth and respiration rates. Phenotipically, mutants plants are indistinguishable from wild type plants. Interestingly, AtãCA2 present several transcription factor features and is highly expressed in flowers. In this work, we study mRNA levels of several nuclear genes encoding mitochondrial proteins by RT-PCR in different backgrounds (mutant, double mutant and AtãCA2 overexpressing plants). Results suggest that AtãCA2 could regulate gene expression of nuclear complex I genes mainly in flowers. Hence, the lower levels of complex I could be attributed to the specific function of AtãCA2 as a transcription factor involved in retrograde regulation.ãca2 and atãca3 plants revealed that complex I levels are diminished about 80% and 10%, respectively. Cell cultures show lower growth and respiration rates. Phenotipically, mutants plants are indistinguishable from wild type plants. Interestingly, AtãCA2 present several transcription factor features and is highly expressed in flowers. In this work, we study mRNA levels of several nuclear genes encoding mitochondrial proteins by RT-PCR in different backgrounds (mutant, double mutant and AtãCA2 overexpressing plants). Results suggest that AtãCA2 could regulate gene expression of nuclear complex I genes mainly in flowers. Hence, the lower levels of complex I could be attributed to the specific function of AtãCA2 as a transcription factor involved in retrograde regulation.ãCA2 present several transcription factor features and is highly expressed in flowers. In this work, we study mRNA levels of several nuclear genes encoding mitochondrial proteins by RT-PCR in different backgrounds (mutant, double mutant and AtãCA2 overexpressing plants). Results suggest that AtãCA2 could regulate gene expression of nuclear complex I genes mainly in flowers. Hence, the lower levels of complex I could be attributed to the specific function of AtãCA2 as a transcription factor involved in retrograde regulation.ãCA2 overexpressing plants). Results suggest that AtãCA2 could regulate gene expression of nuclear complex I genes mainly in flowers. Hence, the lower levels of complex I could be attributed to the specific function of AtãCA2 as a transcription factor involved in retrograde regulation.ãCA2 could regulate gene expression of nuclear complex I genes mainly in flowers. Hence, the lower levels of complex I could be attributed to the specific function of AtãCA2 as a transcription factor involved in retrograde regulation.ãCA2 as a transcription factor involved in retrograde regulation.