INTERACTION STUDIES BETWEEN FIVE MITOCHONDRIAL -CARBONIC ANDHYDRASES FROM ARABIDOPSIS THALIANA

MARTIN, M. V.; VILLARREAL, F.; EDUARDO JULIAN ZABALETA

Pinamar

Congreso; PABMB 10th congress; 2005

A novel group of 5 ¥ã-Carbonic anhydrases (¥ã- CA1, ¥ã- CA2, ¥ã- CA3, ¥ã- CAL1 y ¥ã- CAL2), has been recently identified in¥ã-Carbonic anhydrases (¥ã- CA1, ¥ã- CA2, ¥ã- CA3, ¥ã- CAL1 y ¥ã- CAL2), has been recently identified in¥ã- CAL1 y ¥ã- CAL2), has been recently identified in Arabidopsis thaliana. These proteins are targeted to mitochondria, and they are specifically located bounded mainly to the Complex I (NADH-ubiquinone oxidorreductase) of the mitochondrial electron transport chain. The ¥ã-Carbonic anhydrase proteins containing lefthanded parallel beta helix (L©¬H) domain display imperfect tandem repeated copies of hexapeptide sequence characterized as [LIV]- [GAED]-X2-[STAV]-X. This domain is involved in protein-protein interaction. There is only one example fully characterized of ¥ã- CA from Methanosarcina thermophila, named CAM. Cristallografic studies of this protein shown a quaternary homotrimeric structure coordinating a Zinc ion. In the present work we postulate a plant CA complex by in vivo Yeast two hybrid assays.. These proteins are targeted to mitochondria, and they are specifically located bounded mainly to the Complex I (NADH-ubiquinone oxidorreductase) of the mitochondrial electron transport chain. The ¥ã-Carbonic anhydrase proteins containing lefthanded parallel beta helix (L©¬H) domain display imperfect tandem repeated copies of hexapeptide sequence characterized as [LIV]- [GAED]-X2-[STAV]-X. This domain is involved in protein-protein interaction. There is only one example fully characterized of ¥ã- CA from Methanosarcina thermophila, named CAM. Cristallografic studies of this protein shown a quaternary homotrimeric structure coordinating a Zinc ion. In the present work we postulate a plant CA complex by in vivo Yeast two hybrid assays.¥ã-Carbonic anhydrase proteins containing lefthanded parallel beta helix (L©¬H) domain display imperfect tandem repeated copies of hexapeptide sequence characterized as [LIV]- [GAED]-X2-[STAV]-X. This domain is involved in protein-protein interaction. There is only one example fully characterized of ¥ã- CA from Methanosarcina thermophila, named CAM. Cristallografic studies of this protein shown a quaternary homotrimeric structure coordinating a Zinc ion. In the present work we postulate a plant CA complex by in vivo Yeast two hybrid assays.¥ã- CA from Methanosarcina thermophila, named CAM. Cristallografic studies of this protein shown a quaternary homotrimeric structure coordinating a Zinc ion. In the present work we postulate a plant CA complex by in vivo Yeast two hybrid assays.Methanosarcina thermophila, named CAM. Cristallografic studies of this protein shown a quaternary homotrimeric structure coordinating a Zinc ion. In the present work we postulate a plant CA complex by in vivo Yeast two hybrid assays.in vivo Yeast two hybrid assays.