INVESTIGADORES
ZABALETA Eduardo Julian
congresos y reuniones científicas
Título:
INDUCTION OF GENES INVOLVED IN ANAPLEROTIC PATHWAYS IN MALE STERILE A. THALIANA
Autor/es:
LOBATO, M. E. G.; EDUARDO JULIAN ZABALETA; ARAYA, A; GÓMEZ-CASATI, D.; BUSI, V.
Lugar:
Rosario
Reunión:
Congreso; 42th Raunion Anual de SAIB; 2006
Resumen:
Mitochondrial retrograde signaling is a pathway of
communication from mitochondria to nucleus under normal and
abnormal conditions. It involves multiple factors that sense and
transmit mitochondrial signals to make changes in nuclear gene
expression. These changes lead to a reconfiguration of the
metabolism to accommodate cells to defects in mitochondria. To
study the effect of a mitochondrial dysfunction induced by the
expression of the unedited form of the subunit 9 of ATP synthase
gene (u-ATP9) in Arabidopsis, we constructed transgenic plants
expressing u-ATP9 under the control of two different floral
promoters: Apetala3 and A9. Both plants expressing u-ATP9
exhibited a male sterile phenotype. Using microarrays, we
analyzed the transcriptome changes in Arabidopsis AP3- and A9-
u-ATP9 vs. wt plants. We found altered levels of several genes
codifying for enzymes involved in glycolysis and Krebs cycle,
such as two up-regulated malate dehydrogenases (At5g43330
and At1g04410), and down-regulation of a pyruvate
decarboxylase (At5g54960). In agreement with the microarray
data, we confirmed these results by RT-PCR and also correlate
with changes in their enzymatic activity. This differential activity
seems to enhance the production of energy under reduced pO2
as a hallmark of the hypoxic response.
figuration of the
metabolism to accommodate cells to defects in mitochondria. To
study the effect of a mitochondrial dysfunction induced by the
expression of the unedited form of the subunit 9 of ATP synthase
gene (u-ATP9) in Arabidopsis, we constructed transgenic plants
expressing u-ATP9 under the control of two different floral
promoters: Apetala3 and A9. Both plants expressing u-ATP9
exhibited a male sterile phenotype. Using microarrays, we
analyzed the transcriptome changes in Arabidopsis AP3- and A9-
u-ATP9 vs. wt plants. We found altered levels of several genes
codifying for enzymes involved in glycolysis and Krebs cycle,
such as two up-regulated malate dehydrogenases (At5g43330
and At1g04410), and down-regulation of a pyruvate
decarboxylase (At5g54960). In agreement with the microarray
data, we confirmed these results by RT-PCR and also correlate
with changes in their enzymatic activity. This differential activity
seems to enhance the production of energy under reduced pO2
as a hallmark of the hypoxic response.