INVESTIGADORES
ZABALETA Eduardo Julian
artículos
Título:
Arabidopsis thaliana Embryo Sac Mitochondrial Membrane Potential Stain
Autor/es:
MARTIN, M. V.; FIOL D; EDUARDO JULIAN ZABALETA; PAGNUSSAT G
Revista:
Bio-protocols
Editorial:
www.bio-protocol.org
Referencias:
Lugar: Stanford; Año: 2014
ISSN:
2331-8325
Resumen:
The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. JC-1 is a lipophilic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial potential, JC-1 spontaneously forms complexes with intense red fluorescence. On the other hand, in mitochondria with low mitochondrial potential, JC-1 remains in the monomeric form, which exhibits only green fluorescence.
