INVESTIGADORES
ZABALETA Eduardo Julian
artículos
Título:
Recombinant plant gamma CA2 homotrimers binds bicarbonate
Autor/es:
MARTIN, M. V.; VILLARREAL, F.; MIRAS, I.; HAOUZ, A.; NAVAZA, A.; GONZALEZ LEBRERO R; KAUFMAN S; ZABALETA, EJ
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2009 vol. 583 p. 3425 - 3430
ISSN:
0014-5793
Resumen:
Gamma carbonic anhydrases (cCA) are widespread in Prokaryotes. In Eukaryotes, homologous geneswere found only in plant genomes. In Arabidopsis and maize, the corresponding gene products aresubunits of mitochondrial Complex I.At present, only cCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbondioxide (CO2) hydration activity. In the present work, it is shown that recombinant plant cCA2 couldform homotrimers and bind H14CO3 . However, they are unable to catalyse the reversible hydrationof CO2.These results suggest that plant cCAs do not act as carbonic anhydrases but with a related activitypossibly contributing to recycle CO2 in the context of photorespiration.
