Correlation between PMCA activity and tubulin on platelet function in spontaneously hypertensive rats

ZANOTTO S; MULHBERGER T; BALACH M; A CAMPETELLI; C H CASALE.; MONESTEROLO NE,

Congreso; saib 2016; 2016

Inprevious work we have shown that tubulin and PMCA form a complex, and theformation of this complex regulates the PMCA activity in normotensive andhypertensive subjects. It has previously been reported that increased calciumintraplatelet  can cause hyperactivationof platelets during hypertension. Because of the importance of calcium regulation inplatelets we decided to study the regulation of PMCA by tubulin in hypertensiverats. We observed a significant increase in acetylated and detyrosinatedtubulin and presence of complex of tubulin and PMCA, suggesting the interactionboth proteins. Analysis the PMCA activity revealed thatthe activity in platelets hypertensive rats is decreased by 50% compared to thecontrol, and this decrease in activity correlates with a higher degree ofphosphorylation on tyrosine residues in PMCA. Functional analysis of plateletsobtained by measurement of bleeding time indicates that in hypertensive ratsthe time to form platelet plug is 3 times less than that needed in Wistarrats.  In summary would indicate acorrelation between the high tubulin content, low PMCA activity and higheractivation in platelets hypertensive rats.