Regulation of aldose reductasa by tubulina: effecto on the arterail hypertension and erythrocyte deformabilitty in diabetic rats.

RIVELLI JF; MONESTEROLO NE,; SANTANDER V; OCHOA AL; ARCE C; C CASALE

Rosario

Congreso; SAIB 2015; 2015

SAIB

The main pathogenic pathway of diabetes involved inthe development of chronic complications of this disease is activation of theenzyme aldose reductase (AR). In our laboratory we describe a new regulatorymechanism of AR activity by tubulin interaction. The tubulin increased 7 timesthe AR activity. This activation induces changes in microtubule dynamics,inhibition of Na+,K+-ATPase and prevent the diabetic cataracts in rats. We alsodemonstrate that 3-nitro-L-tyrosine is able to prevent tubulin / AR associationand abolish the activation of the AR by tubulina. The aim of this study was toevaluate the effect of 3-nitro-L-tyrosine on the arterial hypertension anddeformability of erythrocytes in diabetic rats, which they correlated withhyperactivation of AR. The results indicate that administration of3-nitro-L-tyrosine decrease the arterial hypertension and increase theerythrocyte deformability in diabetic rats. This allows us to speculate thatthe tubulin / HR association is not only involved in the formation of diabeticcataract but also in the deformability of the erythrocytes and from there tothe blood pressure.