OSMOTIC FRAGILITY IN GLUCOSE-TREATED OR DIABETIC ERYTHROCYTES IS MEDIATED BY ACETYLATED TUBULIN

NIGRA AD, MONESTEROLO NE, AMAIDEN MR,; C H CASALE.; RIVELLI JF, SANTANDER VS

ROSARIO

Congreso; SAIB 2014; 2014

SAIB

In previous work we demonstrated that high glucose concentration induces translocation of tubulin from sedimentable fraction to the plasma membrane in erythrocytes from diabetic subjects and this change affected cellular deformability. In this work osmotic fragility of erythrocytes was analyzed. We observed a significant increase of erythrocyte osmotic fragility in diabetic subjects and a similar result was observed in erythrocytes from control subjects treated with high glucose concentrations. Due to glucose induces increases in acetylated tubulin levels and its translocation to the membrane in erythrocytes; we evaluated the effect of acetylated tubulin levels on osmotic fragility. The erythrocytes pretreated with trichostatin A, to increase acetylated tubulin levels, and then incubated for 3 hours with glucose have an increased hemolysis compared to those that were only treated with glucose. Following acetylation capacity was analyzed in each case. The results suggest that glucose decreases de deacetylation capacity, which was corroborated by treating erythrocytes with tubacin as a specific inhibitor of HDAC6. This work allows us to conclude that the increased level of acetylated tubulin in erythrocyte membrane affects its properties and that this increase occurs in diabetics by the decrease in deacetylation capacity.