CHANGES IN TUBULIN TYROSINE LIGASE OF ERYTHROCYTES DURING ESTABLISHMENT OF HYPERTENSION

SANTANDER VS, MONERRIS GJ, NIGRA AD, AMAIDEN MR, MONESTEROLO NE, CAMPETELLI AN,; C H CASALE.

ROSARIO

Congreso; SAIB 2014; 2014

SAIB

In previous work we demonstrated that Tubulin Tyrosine Ligase (TTL) activity is reduced in human erythrocytes from hypertensive subjects. In this work content, distribution and activity of this enzyme was evaluated before and after development of hypertension in spontaneously hypertensive rats (SHR). Since hypertension develops after 8-weeks old in SHR these parameters were evaluated in 4 and 12-weeks old rats. After hypertension establishment, TTL activity was 50 % lower in erythrocytes from SHR than Wistar, which were normotensive controls. In according, Western blot analysis shows that TTL content was lower in erythrocytes from than normotensive ones. Distribution of TTL along the cell was also different between both groups. In opposite, before hypertension is established, neither activity nor content of TTL were statistically different between erythrocytes from SHR and Wistar rats. Moreover, TTL activity was modified in other tissues, such heart, kidney and brain, which are relevant in the hypertension development. In conclusion, results suggest that during establishment of hypertension changes in TTL content and activity in different tissues are induced, since detyrosinated tubulin, substrate of TTL, is involved in NKA regulation, this changes could be involved in alteration of NKA activity observed in hypertensive subjects.