INCREASED OF HUMAN ERYTHROCYTES DEFORMABILITY BY DIACYLGLICEROL AND PHOSPHATIDIC ACID

MONESTEROLO NE,; AMAIDEN MR; SANTANDER V.; ARCE C; CASALE C

Mendoza

Congreso; SAIB 2012; 2012

SAIB

During the hypertension numerous alterations of the rheological properties of the blood have been described, such as erythrocytes deformability. This parameter is determined partially by the viscoelastic properties of cell membrane, which depend on his lipid composition and the intracellular calcium concentration. Previously, we demonstrated that the content of tubulin in the membrane affects the deformability of erythrocytes. On the other hand, we have described that plasma membrane calcium ATPase (PMCA), a P-ATPase, form a complex with tubulin, as a consequence enzymatic activity results inhibited; this regulation is dependent on the lipidic environment where PMCA is immersed. On the base of these antecedents, in the present work we study if changes in de lipidic environments are able to affect erythrocytes deformability in normotensive and hypertensive subjects. Results shown that the addition of 50 M of DAG or PA increases erythrocyte deformability in normotensive and hypertensive subjects. Moreover, after lipid treatment are not observed significantly differences in the deformability of erythrocytes of normotensive and hypertensive subjects. The increase in the erythrocyte deformability would be mediated by a mechanism in which lipids, tubulin and P-ATPases are involved.