Effects of detyrosinated tubulin on Na(+),K(+)-ATPase activity and erythrocyte function in hypertensive subjects.

AMAIDEN MR; SANTANDER V; MONESTEROLO NE,; A NIGRA; RIVELLI JF; CAMPETELLI A.N; PIE-JUSTE J; C H CASALE.

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 589 p. 364 - 373

0014-5793

Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. Moreover, we report a reduced content and activity of the enzyme tubulin tyrosine ligase in erythrocytes of hypertensive subjects. Such alterations are related to changes in erythrocyte deformability. Our findings indicate that the detyrosination/tyrosination cycle of tubulin is important in regulation of NKA activity, and that abnormalities in this cycle are involved in hypertension development.