INVESTIGADORES
CASALE Cesar Horacio
artículos
Título:
Cholinesterase, Acid Phosphatase, and Phospholipase C of Pseudomonas aeruginosa Under Hiperosmotic Conditions in a High-Phosphatase Medium
Autor/es:
LISA AT; CASALE CH; DOMENECH CE
Revista:
CURRENT MICROBIOLOGY
Referencias:
Año: 1994 vol. 28 p. 71 - 76
ISSN:
0343-8651
Resumen:
Abstract. The presence of low choline or betaine concentrations in a culture medium containing
succinate, NH4CI, and inorganic phosphate (Pi) as the carbon, nitrogen, and phosphate sources,
respectively, permits the growth of Pseudomonas aeruginosa in a hyperosmolar medium. Dimethylglycine,
acetylcholine, and phosphorylcholine were less effective as osmoprotectants
than choline or betaine. Other alkylammonium compounds tested were virtually ineffective in
this capacity. Bacterial growth was also observed in a hyperosmolar medium when choline
was the sole carbon and nitrogen source. Choline could act as an osmoprotectant under all the
conditions tested. However, the production of cholinesterase (ChE), acid phosphatase
(Ac.Pase) and phospholipase C (PLC) took place only when choline was the carbon and nitrogen
source. This fact confirms that the synthesis of PLC may occur even in the presence of a
high Pi concentration in the medium. Inasmuch as in a high-Pi medium the synthesis of PLC and
Ac.Pase (phosphorylcholine phosphatase) is dependent only on choline metabolism, it is postulated
that both enzymes are involved in a set of reactions coordinated to produce the breakdown
of the membrane phospholipids of the host cell in a hyperosmotic medium.Pseudomonas aeruginosa in a hyperosmolar medium. Dimethylglycine,
acetylcholine, and phosphorylcholine were less effective as osmoprotectants
than choline or betaine. Other alkylammonium compounds tested were virtually ineffective in
this capacity. Bacterial growth was also observed in a hyperosmolar medium when choline
was the sole carbon and nitrogen source. Choline could act as an osmoprotectant under all the
conditions tested. However, the production of cholinesterase (ChE), acid phosphatase
(Ac.Pase) and phospholipase C (PLC) took place only when choline was the carbon and nitrogen
source. This fact confirms that the synthesis of PLC may occur even in the presence of a
high Pi concentration in the medium. Inasmuch as in a high-Pi medium the synthesis of PLC and
Ac.Pase (phosphorylcholine phosphatase) is dependent only on choline metabolism, it is postulated
that both enzymes are involved in a set of reactions coordinated to produce the breakdown
of the membrane phospholipids of the host cell in a hyperosmotic medium.