PD-1+Tim-3+ T cells from 4T1 Tumor bearing mice display Features of exhaustion and increased expression of immunomodulatory molecules?

CANALE F; RAMELLO MC; GOROSITO SERRAN M; ARAUJO-FURLAN C; TOSELLO BOARI J; ACOSTA RODRIGUEZ EV; GRUPPI A; MONTES CL

Mar del Plata

Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica. LXII Reunión Anual de la Sociedad Argentina de Inmunologia; 2014

SAI-SAIC

CHARLAS CORTAS SELECCIONADAS: PD-1+TIM-3+ T CELLS FROM 4T1 TUMOR-BEARING MICE DISPLAY FEATURES OF EXHAUSTION AND INCREASED EXPRESSION OF IMMUNOMODULATORY MOLECULES. T cell exhaustion is a T cell dysfunction state that arises during chronic infections and cancer. It is characterized by poor effector functions and sustained expression of inhibitory receptors (iRs) like PD-1 and TIM-3. Based on our previous findings, we hypothesize that these apparently dysfunctional T cells may have modulatory functions. In this work we studied phenotype and functional characteristics of tumor-infiltrating lymphocytes (TILs) and T cells from tumor-draining lymph nodes (dLN) of 4T1 mammary carcinoma bearing mice; comparing PD-1+TIM-3+ double positive (DP) cells with PD-1-TIM-3- double negative (DN) cells. Within DP TILs population we found higher % of cells expressing iRs like 2B4, LAG-3 and KLRG-1 compared to DN T cells, being 2B4 and LAG-3 more frequent in CD8+ DP T cells (p