Ursodeoxycholate (UDC) prevents MRP2 activity impairment in ethinylestradiol (EE)-induced cholestasis.

SANCHEZ POZZI; D'ANDREA, VANESA; CROCENZI, FERNANDO A; PELLEGRINO, JOSÉ M; LUQUITA, MARCELO G; CATANIA, VIVIANA A

Salvador, Bahia

Congreso; Bianual Meeting of the IASL; 2004

International Association for the Study of the Liver

EE administration induces cholestasis by affecting both bile salt-dependent and bile salt-independent fractions of the bile flow. The decrease in bile salt-independent flow is thought to be due, in part, to a reduction in the expression of the canalicular transporter Mrp2. UDC administration reverts estrogen-induced decrease in bile flow, but the effect of UDC on Mrp2 in this model of cholestasis has not been analyzed as yet. The aim of this study was to analyze the protective effect of UDC on EE-induced impairment of Mrp2 activity, using the model substrate dinitrophenyl-glutathione (DNP-SG) to evaluate transport in vivo and in isolated hepatocytes. Western blot analysis was used to evaluate protein content