INVESTIGADORES
SANCHEZ POZZI Enrique Juan
artículos
Título:
Quantitative and qualitative gender-related differences in jejunal-glutathione S-transferase in the rat. Effect of testosterone administration
Autor/es:
A CATANIA, VIVIANA; G LUQUITA, MARCELO; ENRIQUE JUAN SANCHEZ POZZI; D MOTTINO, ALDO
Revista:
LIFE SCIENCES
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2000 vol. 84 p. 467 - 474
ISSN:
0024-3205
Resumen:
Gender-related differences and the regulation by testosterone of glutathione S-transferase were studied in rat
jejunum. We analyzed enzyme activity and the relative content of GST
subunits. Four experimental groups of adult rats were studied: normal
males, castrated males, castrated males injected with testosterone and normal females. Glutathione S-transferase activity was assayed using 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Differences
in subunit composition among groups were evaluated by western blot
analysis. The results demonstrated that 1-chloro-2,4-dinitrobenzene
conjugation rate is higher in normal males than in normal females and
castrated males. Testosterone administration to castrated males raised the activity up to the level observed in normal males. No significant difference in glutathione S-transferase
activity towards 1,2-dichloro-4-nitrobenzene was observed among groups.
Western blot analysis revealed that males and females differ in all
subunits tested that is, rGSTA2, rGSTM1, rGSTM2 and rGSTP1, and that testosterone regulates the content of rGSTM1, rGSTM2 and rGSTP1. In conclusion, jejunal GST shows a gender-dependent regulation affecting both enzyme activity and subunit composition, and testosterone appears to be one of the factors involved.